Extended Data Fig. 2: Processing scheme and resolution information of the Rho-2TMD map.

a, Flowchart depicting the classification scheme and analysis of cryo-EM micrographs of the Rho-2TMD RNC sample. The classes denoted as “TRAP complex” appear to only contain the core translocon comprised of the Sec61 and TRAP complexes, evidently in somewhat different conformations, as characterised previously in cryo-EM and cryo-electron tomography work^26,61,62,63. These were not pursued further. Note that model building relied almost entirely on the Rho-2TMD reference map, with a few regions employing the two submaps for verification. The composite map shown in Fig. 2a is solely for illustration purposes as a means of simultaneously displaying the best regions contributed by each map. FCwSS indicates focused classification with signal subtraction. b, Fourier shell correlation (FSC) curve for the Rho-2TMD reference map illustrating an overall resolution of 3.25 Å by the gold-standard method⁶⁴. c, Two views of the Rho-2TMD reference map coloured by local resolution. Key structural elements are indicated.