Extended Data Fig. 9: Recovery from depression is slowed by loss of Syt3 at cerebellar mossy fibre synapses.
From: Fast resupply of synaptic vesicles requires synaptotagmin-3
a) Schematic of cerebellar mossy fibre recordings. EPSCs were recorded from voltage-clamped granule cells (GC) while mossy fibres (MF) were activated by electrical stimulation in the white matter 50-100 μm from the cell. b) Representative EPSCs elicited by 100 stimuli at 200 Hz, followed by stimuli at varying intervals to probe recovery from depression (ΔtRecovery). c-d) Time course of recovery of EPSCs in the first 0.5 s after trains (c, linear scale) and 10 s (d, logarithmic scale). e) Weighted time constants of biexponential recovery (τw) (P = 0.01). Data are mean ± s.e.m. Number of experiments are shown in Extended Data Table 1. Statistical significances are shown as *: P < 0.05. Significances were evaluated using two-tailed Student’s t-tests.
