Extended Data Fig. 10: Recovery from depression is not affected in Syt7 KOs at calyx of Held or climbing fibre synapses.
From: Fast resupply of synaptic vesicles requires synaptotagmin-3
a) Representative EPSCs elicited by 100 stimuli at 200 Hz, followed by stimuli at varying intervals to probe recovery from depression (ΔtRecovery) at the calyx of Held in WT (black) and Syt7 KO (red) synapses. b) First second of recovery of EPSCs after 200 Hz stimulation, fit with biexponential curves. c) Full time-course of recovery of EPSCs after 200 Hz stimulation. d) Weighted time constant of biexponential recovery (τw) for both genotypes (P = 0.40). e) Superimposed recordings of EPSCs evoked by pairs of stimuli of climbing fibre synapses with varying ΔtRecovery. f-g) Time-course of recovery from depression in linear (f) and logarithmic scale (g). h) Weighted time constant of biexponential recovery (τw) for both genotypes (P = 0.14). WT data were reproduced from Figs. 2 & 4. Data are mean ± s.e.m. Number of experiments are shown in Extended Data Table 1. Significances were evaluated using Kruskal-Wallis tests (d) and two-tailed Student’s t-tests (h). Critical significance thresholds were post hoc Šidák corrected.
