Extended Data Fig. 13: SYT3 contributes to facilitation at cerebellar parallel fibre synapses.
From: Fast resupply of synaptic vesicles requires synaptotagmin-3
a) Schematic of cerebellar parallel fibre recordings. EPSCs were recorded from voltage-clamped Purkinje cells (PC) while parallel fibres were activated by electrical stimulation in the molecular layer. b) Superimposed recordings of EPSCs evoked by pairs of stimuli at the at varying intervals (Δt) in WT (black), Syt3 KO (magenta) and Syt7 KO (red) synapses. c) Average paired-pulse ratios fit exponentially. d) Average paired-pulse ratios at Δt = 5 ms and time constant of exponential fits to data in b. e) Representative EPSCs evoked by 20 stimuli at 50 Hz. f) Average normalized EPSCs during 50 Hz stimulation. Magenta and red bars indicate significant differences between WT and Syt3 KO, or WT and Syt7 KO, respectively. Gray bar indicates significant differences between Syt3 KO and Syt7 KO. g) Normalized amplitude of the last 5 EPSCs (EPSC16-20) during 50 Hz stimulation (WT vs. Syt3 KO: P = 0.0002; WT vs. Syt7 KO: P = 0.009; Syt3 KO vs. Syt7 KO: P = 0.007). Data are mean ± s.e.m. Number of experiments are shown in Extended Data Table 1. Statistical significances are shown as *: P < 0.05, **: P < 0.01, ***: P < 0.001. Significances were evaluated using one way ANOVA followed by two-tailed Student’s t-tests. Critical significance thresholds were post hoc Šidák corrected.
