Extended Data Fig. 6: Delayed requirement of the AC activity in root growth regulation.
From: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
a, Loss of AC activity does not affect cvxIAA/ccvTIR1-induced root growth inhibition within the first hour. A vRootchip experiment was performed with the transgenic lines indicated, and the images were captured with a time interval of 1 min. Mock medium was changed to medium containing 500 nM of cvxIAA at 40 min. Root growth rate was normalized to the starting point of the respective group. The values shown are means + SD. n = 4 or 5 seedlings. The experiment was repeated twice independently with similar results. b, Resistance of ccvTIR1ACm1 to cvxIAA-triggered root growth inhibition occurs only after 1 h of treatment. Vertical scanner growth assay was performed to track the root growth dynamics. Five-days-old seedlings of the indicated genotypes were transferred to either Mock medium or medium containing 200 nM of cvxIAA. Images were taken every 30 min. Root growth rate was measured. The values shown are means + SD. n = 10 seedlings. The experiment was repeated twice independently with similar results.
