Extended Data Fig. 7: TIR1 AC activity is not crucial for rapid auxin responses.
From: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
a, cvxIAA triggers similar Ca2+ spikes in the ccvTIR1 and ccvTIR1ACm1 lines. The calcium sensor GCaMP3 was crossed to the indicated transgenic lines. Five-days-old F1 seedlings were used for vRootchip experiment, and the images were captured with a time interval of 15 s. Mock medium was changed to medium containing 500 nM of cvxIAA at 10 min. The fluorescence signal in the epidermal cells of root elongation zone was quantified, and was normalized to the average value of time points before treatment. The values shown are means + SD. n = 4 seedlings. The experiment was repeated twice independently with similar results. b, Auxin-induced apoplastic alkalinisation is not changed in the TIR1ACm1 line. Five-days-old seedlings of the indicated genotypes were used for vRootchip experiment. Mock medium was changed to medium containing 10 nM of IAA at 11 min. Ratiometric (488 nm/405 nm) imaging of HPST staining was used to measure apoplastic pH in the epidermal cells of root elongation zone. The values shown are means + SD normalized to the average of those time points before treatment. n = 3 or 4 seedlings. The experiment was repeated twice independently with similar results.
