Extended Data Fig. 3: AC activity is conserved in TIR1/AFBs orthologues from Physcomitrella.
From: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
a, Alignment of the C-terminal protein sequences of TIR1/AFBs together with their orthologues from Physcomitrella. Note the AC motif is highly conserved in all the sequences. Only the first amino acid is a bit more relaxed. b, TIR1/AFBs orthologues from Physcomitrella have AC activity. GST-tagged PpAFBs were purified from E. coli. In vitro AC activity assay was performed with GST as the negative control. cAMP level after reaction was quantified using LC-MS/MS. The values shown are means ± SD from 3 biological replicates. One-way ANOVA and Dunnett’s multiple comparisons test were performed. Asterisks indicate significant difference between the corresponding group and the negative control (GST). ** p ≤ 0.01 (p = 0.0034); **** p ≤ 0.0001.
