Extended Data Fig. 6: Structural conservation between SEACIT and GATOR1.
a, Comparison between the trimeric Sea1-Npr2-Npr3 complex (SEACIT) and GATOR1 (PDB:7t3b). b, Comparison of the Npr2-Npr3 heterodimer (left) with Nprl2-Nprl3 (right). c, d, Sea1 interacts with Npr2 through its SABA domain, using an extension to interact with the C-lobe. e, Rapamycin growth assays (5 nM) of SEACAT and SEACIT deletion strains. Deletions of SEACIT subunits cause a slight increase in rapamycin resistance, whereas deletions of SEA2 and SEA4 cause hypersensitivity to rapamycin. SEA3 deletion causes an intermediate SEACIT-SEACAT phenotype. e, Removal of the Sea3SAP is sufficient to induce a SEACAT-like phenotype.
