Extended Data Fig. 5: Anion currents from hBest2 variants and the influence of hGS.
From: Bestrophin-2 and glutamine synthetase form a complex for glutamate release
(a-c) Bar chart showing the steady-state current densities at +100 mV from HEK293 cells transiently expressing the indicated hBest2 and hGS constructs with 1 μM [Ca2+]i under different conditions: (a) Cl− as the principal internal and external anion; n = 9, 5, 7, 7, 5, 8, 5 and 6 for the groups from left to right, respectively; *p = 0.04, 7E-3, 2E-3 and 2E-3 for the groups from left to right, respectively, compared to currents from cells expressing WT hBest2 alone. (b) Internal glutamate and external Cl− as the principal anions; n = 5, 8, 9, 11, 7, 11, 5 and 6 for the groups from left to right, respectively; *p = 3E-4, 9E-4, 0.01 and 0.02 for the groups from left to right, respectively, compared to currents from cells expressing WT hBest2 alone. (c) Internal Cl− and external glutamate as the principal anions; n = 6, 6, 12, 7, 6 and 8 for the groups from left to right, respectively; *p = 5E-4, 6E-6 and 0.047 for the groups from left to right, respectively, compared to currents from cells expressing WT hBest2 alone. All error bars are presented as mean values +/− SEM; p values are calculated by two-tailed unpaired Student’s t test.
