Extended Data Fig. 2: Bath application and injection of sterile saline 0.9% do not impact hippocampal activity and behavior.
From: Entorhinal cortex directs learning-related changes in CA1 representations
a—e, Comparison of bath application (black, n = 5 animals) and injection (red, n = 5 animals) of sterile saline 0.9% in environment A. a, Left: Fraction of CA1 cells that are spatially modulated (two-tailed unpaired t-test, bath vs. injection, p = 0.628). Right: Mean place cell spatial information content per animal (two-tailed unpaired t-test, bath vs. injection, p = 0.08). b, Fraction of CA1 place cells as a function of place field peak location. The track is divided into ten spatial bins of 18 cm. c, Left. Mean lick rates for bath application and injection of sterile saline 0.9%. Right: Mean number of licks outside the reward zone (from 14 cm before to 36 cm after the reward) divided by the total number of licks (two-tailed unpaired t-test, bath vs. injection, p = 0.467). d, Left: Mean running profile for bath application and injection of sterile saline 0.9%. Right: Minimum velocity inside the reward zone divided by mean velocity outside the reward zone (two-tailed unpaired t-test, bath vs. injection, p = 0.768). e, Time course of CA1 place cell appearance for bath application and injection of sterile saline 0.9%. In panels a, c, d the open circles show individual animals, the filled circles the mean. f—j, Same as a—e for comparing control condition (black, no saline, n = 18 animals) and sterile saline 0.9% application via bath application and injection (red, n = 10 animals) in environment A. Black dashed lines depict the reward location. Data are shown as mean +/− SEM.
