Extended Data Fig. 9: (related to Fig 4): ILC2-deficient mice are highly susceptible to N. brasiliensis infection.

7 (a-h) or 11 days (i—m) after infection with N.b., Nmur1^iCre-GFP Gata3^fl/fl (a,b,f-h) or Nmur1^iCre-eGFP Id2^fl/fl (c-e, i—m) mice (cKO) and littermate controls (Ctrl) were analysed alongside untreated WT controls. a,b, Nmur1^iCre-GFP Gata3^fl/fl mice (cKO) and littermate controls (Ctrl) 7 days after N. b. infection alongside untreated WT controls. ILC2s (a) and eosinophils (b) in the mLN and lung, flow cytometry plots depicting mLN. c, T_H2 cells in mLN and lung. d,e Neutrophils in the lung. Littermate controls for c—e were Id2^fl/+ or Nmur1^iCre-eGFP Id2^fl/+. f, Worm burden g, Goblet- and tuft cells per villus, mucus area quantified from UEA-I stain, all as shown in h. h, left: Periodic acid-Schiff staining; middle: intestinal tuft cell staining with Dclk1 (red), DAPI (blue); right: intestinal mucus stained with UEA-I (green), DAPI (blue). Littermate controls for a,b,f-h were Gata3^fl/+. i,j, ILC2s and eosinophils in the mLN (i) or mLN and lung (j) 11 days after infection. k, T_H2 cells. l, Goblet- and tuft cells per villus, mucus area quantified from UEA-I stain as shown in m. m, intestine as shown in h, 11 days after infection. n-r Infection of BM chimeras of C57BL/6 recipients transplanted with bone marrow of Id2^fl/fl (Ctrl), Nmur1^iCre-eGFP Id2^fl/fl (cKO) donors, or bone marrow of cKO donors supplemented with sorted ILC2 precursors (cKO + ILC2p) from C57BL/6 donors, with N.b. n, Periodic acid-Schiff staining of small intestine seven days after N. b. infection. o, Goblet cells per villus. p,q, Neutrophils in the lung. r, T_H2 cells. s, ILC3s, LTi-like ILC3s and NCR⁺ ILC3s 7 days after N.b. infection of Nmur1^iCre-eGFP Id2^fl/fl mice (cKO) and littermate controls (Ctrl). a—s One symbol represents data from one mouse, data are mean ± s.d. one-way ANOVA with Tukey’s multiple comparisons, NS not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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