Extended Data Fig. 1: The EpiHR gene set marks a defined tumour cell population across CRCs.
From: Metastatic recurrence in colorectal cancer arises from residual EMP1+ cells
a-c, UMAP layout of whole tumours (stroma + epithelium cells) from 7 CRC patients in the KUL dataset. Coloured by (a) gene expression of all high hazard ratio genes (AllHR), (b) tumour microenvironment-specific HR genes (TME-HR), and (c) epithelial-specific HR genes (EpiHR). d, Association between clinical variables and the EpiHR signature in the CRC meta cohort was assessed by fitting a linear model for each variable independently. Technical factors (dataset and centre, as described in extended methods) were included as covariates. Lines show the left and right confidence intervals. n = 1688 patients. e. Kaplan-Meier survival curves indicating relapse-free survival according to EpiHR gene signature expression for CRC patients classified by CMS. Two-sided Wald test. f-g, UMAP layout of 2718 CRC tumour cells from the KUL cohort coloured by f) patient ID and g) expression of the EpiHR signature. h, Heatmap showing Pearson correlation scores in gene expression among EpiHR signature genes in patients from the SMC cohort. Note that most genes belong to one coherent subset (Cluster 1). Gene lists are detailed in Supplementary Table 2. i, UMAP layout of human CRC tumour cells coloured by the expression of genes belonging to Clusters 1, 2, 3 and 4 identified in (h).
