Extended Data Fig. 5: MFG-E8 levels increase with age and cerebral β-amyloidosis in APP23 mice.
From: Medin co-aggregates with vascular amyloid-β in Alzheimer’s disease
Validation of ELISA results presented in Fig. 2 using a, Western blotting of total mouse brain homogenates, brain homogenate ‘supernatant’, and isolated blood vessels of adult (12–13-month-old) and aged (21–24-month-old) APP23 mice (see Methods for details of the isolation protocol). With increasing Aβ levels, APP23 mice show higher MFG-E8 levels in the soluble vascular fraction, confirming ELISA measurements (cf. Fig. 1i). PDGFR-β and α-SMA were used as vascular markers, β-actin was used as the loading control. b, Quantification of Western Blot bands (adult: n = 3f/3m, aged: n = 2f/5m, two-tailed Mann-Whitney test). Blots were repeated at least two times, with equivalent results. Data shown are means ± S.E.M. Uncropped Western Blots are shown in Supplementary Fig. 5.
