Extended Data Fig. 3: Gene and protein expression of iPfSPZ.
From: In vitro production of infectious Plasmodium falciparum sporozoites
a. Scatter plot showing correlation of transcriptomes; x axis mPfSPZ, y axis iPfSPZ, right upper corner in red print, expressed liver stage genes. b. Heatmap of genes expressed in PfSPZ detected by RNASeq. 3,656 genes with ≥5 TPM in at least one sample are plotted. The raw gene expression reads from both iPfSPZ and mPfSPZ samples are in Supplementary Table 4. c. Comparative transcriptomics of mPfSPZ and iPfSPZ. Volcano plot of differentially expressed genes are plotted based upon average fold change and unadjusted average p-value from the 5 DE tools (significance of differential expression, see methods). Extreme low p-values are cropped at 1e-08 for plotting. Notable, differentially expressed genes are highlighted in red. d. PfCSP expression by mPfSPZ and iPfSPZ by immunoblot analysis. Lanes 1-3 are 2.0x105, 5.0x104 and 1.25x104 mPfSPZ, and lanes 5-7 are 1.1x106, 2.0x105, 5.0x104 iPfSPZ respectively. No sample was loaded in lane 4. Molecular size markers are indicated on the left.
