Extended Data Fig. 10: Control of checkpoint responses by MYC multimers.
From: MYC multimers shield stalled replication forks from RNA polymerase
a. Immunoblot assessing phosphorylation state or RPA32 at S4/S8 of U2OSMYC-Tet-On cells upon treatment with DOX (1 µg/ml, 24 h), HU (3.5 mM, 8 h) and BI8626 (10 µM, 8.5 h). Chromatin-bound proteins shown with histone H3 as loading control (n = 3). For gel source data, see Supplementary Fig 1. b. Spike normalized reads from pRPAS4/S8 CUT&RUN experiment centered around 4,252 common peaks called around promoters in U2OSMYC-Tet-On cells treated with DOX (1 µg/ml, 24 h), HU (3.5 mM, 8 h) and BI8626 (10 µM, 8.5 h) (n = 1). c. Box plots documenting levels of pS33RPA (Left) and pS4/S8RPA (right) in U2OSMYC-Tet-On cells treated as indicated (DOX: 1 µg/ml, 24 h; HU: 5 mM, 4 h; MG-132: 20 µM, 4 h) Box plots are defined as in Fig. 3g. Data were analyzed from n = 946 cells per condition analyzed over three biological independent experiments. d. Model summarizing our findings. In above panels, unless indicated otherwise, n indicates the number of independent biological replicates.
