Extended Data Fig. 1: Ultralong-term activity recordings of the V-ATPase in single SVh.
From: Regulation of the mammalian-brain V-ATPase through ultraslow mode-switching
Activity recordings that spanned the duration of three hours revealed mode-switching of single V-ATPases. Each liposome can contain up to hundreds of fluorophores and the system has been optimized for minimal photon budget usage, therefore minimizing photobleaching. a–f, Left panels: Representative single molecule acidification kinetics displaying long-lived proton pumping and inactive modes. Activity was initiated upon addition of ATP. Red traces correspond to active vesicles that have been normalized to the baseline. Black traces correspond to inactive vesicles. The data as not been corrected for photobleaching. A mild linear filter has been used in the visualization of these traces. Right panels: Histograms of acidification plateaus. Red histograms correspond to active vesicles while black histograms correspond inactive vesicles
