Extended Data Fig. 5: Slice electrophysiology following CSDS, measurement of Ca2+ activity in NTLS neurons responses during food reward and social conditioning.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Characterization of ex vivo electrophysiology parameters measured in NTLS neurons following CSDS in SUS and RES mice. From left to right: Bar graph of average resting membrane potential per neuron (mean SUS= −67.49 mV +/− 0.975 mV, n = 55 with 4–8 neurons/mouse; mean RES = −61.62 mV +/− 1.73 mV, n = 19 with 4–7 neurons/mouse; two-tailed Welch’s t test, P = 0.0059). No difference in action potential threshold (two-tailed Welch’s test, P = 0.3037), amplitude (two-tailed Welch’s test, P = 0.6661), half-width duration (two-tailed Welch’s test, P = 0.3757) or fast after hyperpolarization (fAHP) amplitude (two-tailed Welch’s test, P = 0.7154). b, Social interaction ratio of fiber photometry cohort after CSDS in females (One-Way ANOVA, F (2, 11) = 5.629, P = 0.0207, n = 4 (CTRL), 5 (RES), 5 (SUS)) and males (One-Way ANOVA, F (2, 14) = 12.93, P = 0.0007, n = 7 (CTRL), 5 (RES), 5 (SUS (5)). c, Ca2+ activity in NTLS neurons during consumption of a peanut butter cup (left). Dashed line in c marks the beginning of biting. Comparison of Ca2+ ΔF/F change before and after biting (right, two-tailed paired t test, t = 1.577, df = 4, P = 0.1900, n = 5). d, Ca2+ traces in paired (pink) and unpaired (blue) conditioning chambers. The inset shows normalized area under curve (AUC) between paired and unpaired conditioning sessions (sexes combined, paired two-tailed t-test, CTRL, t = 0.1977, df = 5, P = 0.8511, RES, t = 1.049, df = 5, P = 0.3423, SUS, t = 5.453, df = 5, P = 0.0028), n = 6 per group). ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001. All data are expressed as mean ± s.e.m.
