Extended Data Fig. 6: NTLS neuron manipulation in stress-naïve mice does not change social interaction and CVS has no effect on sCPP.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Expression of AAV-DIO-DREADDs in NTLS neurons. b, NTLS activation in stress-naïve mice does not change social behaviour in females (unpaired two-tailed t-test, t14 = 1.044, P = 0.3141, n = 8 per group) or males (unpaired two-tailed t-test, t14 = 1.434, P =0.3975, n = 8 per group). NTLS activation in stress-naïve mice does not change locomotor activity in females (unpaired two-tailed t-test, t14 = 0.3473, P = 0.7335, n = 8 per group) or males (unpaired two-tailed t-test, t14 = 1.425, P = 0.1762, n = 8 per group). c, NTLS chemogenetic activation in stress-naïve mice does not change social preference (Two-way repeated measures ANOVA, Vehicle, F = (1, 16) = 7.198, P = 0.0163, n = 9, CNO, F (1, 18) = 6.644, P = 0.0190, n = 10). d, Subtracted CPP score comparison between vehicle and CNO group (unpaired two-tailed t-test, t = 0.03518, df = 17, P = 0.9723, n = 10 per group). e, Schematic diagram of CVS and CPP test. f, CVS effect on sCPP in either sex (Two-way repeated measures ANOVA, female, CTRL, F (1, 22) = 4.824, P = 0.0389, n = 12, CVS, F (1, 22) = 5.172, P = 0.0331, n = 12; male, CTRL, F (1, 22) = 5.042, P = 0.0351, n = 12; CVS, F (1, 22) = 3.900, P = 0.0610, n = 12). g, Schematic diagram of virus injection and optogenetic manipulation of NTLS neurons during RTPP test. h, NTLS neuron activation does not alter real time place preference in either stress-naïve females (Paired two-tailed t-test, ChR2, t11 = 0.06179, P = 0.9518, n = 12, EYFP, t15 = 0.01923, P = 0.9849, n = 16) or males (Paired two-tailed t-test, ChR2, t8 = 0.3855, P = 0.7099, n = 9; EYFP, t8 = 0.6333, P = 0.5442, n = 9). ns, not significant. * P < 0.05. All data are expressed as mean ± s.e.m. Scale bar, 100 μm. BioRender was used to generate schematic figures in c,e,g.
