Extended Data Fig. 7: NTLS neurons modulate chronic restraint stress-induced object avoidance and anxiety-like behaviours.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Schematic diagram of chronic restraint stress and RI test. b, Comparison of latency to the first investigation (unpaired two-tailed t-test, t = 3.142, df = 18, P = 0.0056) and number of investigation bouts (t = 5.259, df = 18, P < 0.0001) with a novel restraint tube, or withdrawal from the tube (t = 5.229, df = 18, P < 0.0001), n = 10, between control (CTRL) and chronic restraint stressed (CRS) animals. c, Schematic diagram of CRS with DREADD manipulation during RI test. d, Latency (paired two-tailed t-test, t = 2.065, df = 8, P = 0.0728), and number of investigation bouts (t = 2.619, df = 8, P = 0.0307) with a novel restraint tube, or withdrawal from the tube (t = 1.988, df = 8, P = 0.0820), n = 9. e, Schematic diagram of chronic restraint stress with juvenile bedding/odour (CRSO) and RI test. f, Latency (unpaired two-tailed t-test, t = 0.5452, df = 18, P = 0.5923) and number of investigation bouts (t = 0.3971, df = 18, P = 0.6960) with a novel restrainer tube, or withdrawal from the tube (t = 0.000, df = 18, P > 0.9999), n = 10. g, NTLS activation in stress-naïve male mice leads to higher anxiety-like behaviour in the elevated plus maze (unpaired two-tailed t-test, t14 = 2.824, P = 0.0135, n = 8 per group). h, NTLS activation or inhibition in stress-naïve male mice modulate marble burying behaviour (two-tailed paired t-test, hM3Dq, t7 = 4.631, P = 0.0024, n = 8; hM4Di, t7 = 4.020, P = 0.0051, n = 8). i, NTLS activation or inhibition in stress-naïve male mice leads to higher or lower anxiety levels, respectively, in the open field test (unpaired two-tailed t-test, hM3Dq, t14 = 2.189, P = 0.0461, n = 8 per group; hM4Di, t14 = 1.424, P = 0.1762, n = 8 per group). j, with no locomotor changes (unpaired two-tailed t-test, hM3Dq, t14 = 1.641, P = 0.1230; hM4Di, t14 = 1.566, P = 0.1398). ns, not significant. * P < 0.05, ** P < 0.01, **** P < 0.0001. All data are expressed as mean ± s.e.m. BioRender was used to generate schematic figures in a,c,e,i.
