Extended Data Fig. 8: NTLS monosynaptic downstream region verification.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Schematic diagram of H129ΔTK-TT virus injection. 48 h after injection, there were no tdTomato+ neurons in downstream regions of NTLS neurons. b, Retrograde AAV-DIO-EGFP/tdTomato tracing verification shows NTLS→NAc, NTLS→AHN, NTLS→PAG monosynaptic connections. c, Colocalization analysis for overlapping NTLS projection neurons to the NAc/AHN/PAG. d, Cholera toxin subunit b (CTB) tracing for NTLS→NAc, NTLS→AHN, and NTLS→PAG projections. Representative image of LS (middle panel) with yellow arrowheads indicating neurons projecting to both NAc and AHN. White arrowheads indicate neurons projecting to both PAG and AHN. e, Number of projections showing colocalization of CTB tracers from three downstream regions (3 slices per brain region per mouse, n = 3 mice). All data are expressed as mean ± s.e.m. Scale bar, 100 μm.
