Extended Data Fig. 9: Role of NTLS inputs to NAc and AHN in regulating social behaviours.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Schematic of NTLS targeting with NpHR and manipulation during social behaviour tests. b–e, NpHR axon terminal inhibition in the NAc (b, c) and AHN (d, e) rescued social investigation time and partially rescued social avoidance in both females (b, social investigation, F (1, 14) = 3.484, P = 0.0831, n = 8 per group; social avoidance, F (1, 14) = 1.180, P = 0.2956, n = 8 per group, d, social investigation, F (1, 14) = 4.982, P = 0.0425, n = 8 per group; social avoidance, F (1, 14) = 2.266, P = 0.1545, n = 8 per group) and males (c, social investigation, F (1, 14) = 0.2046, P = 0.6580, n = 8 per group; social avoidance, F (1, 14) = 1.214, P = 0.2891, n = 8 per group, e, social investigation, F (1, 14) = 4.597, P = 0.0501, n = 8 per group; social avoidance, F (1, 14) = 5.359, P = 0.0363, n = 8 per group). f-m, Optogenetic manipulation of NTLS→NAc and NTLS→AHN circuits during social CPP conditioning in both sexes. f, Activation of NTLS→NAc with ChR2 blocked social reward in RES females (EYFP, F (1, 12) = 2.362, P = 0.1502, n = 7, ChR2, F (1, 14) = 0.5543, P = 0.4689, n = 8) g, Inhibition of NTLS→NAc with NpHR rescued social reward deficits in SUS females (EYFP, F (1, 14) = 0.5105, P = 0.4867, NpHR, F (1, 14) = 4.183, P = 0.0601, n = 8 per group). h, Activation of NTLS→AHN with ChR2 blocked social reward in RES females (EYFP, F (1, 12) = 4.289, P = 0.0606, n = 7, ChR2, F (1, 14) = 0.0001296, P = 0.9911, n = 8). i, Inhibition of NTLS→AHN with NpHR rescued social reward deficits in SUS females (EYFP, F (1, 14) = 0.1068, P = 0.7487, NpHR, F (1, 14) = 4.575, P = 0.0505, n = 8 per group). j, Activation of NTLS→NAc with ChR2 blocked social reward in RES males (EYFP, F (1, 12) = 5.703, P = 0.0343, n = 7, ChR2, F (1, 14) = 0.2484, P = 0.6259, n = 8). k, Inhibition of NTLS→NAc with NpHR rescued social reward deficits in SUS males (EYFP, F (1, 14) = 4.053, P = 0.0637, NpHR, F (1, 14) = 4.140, P = 0.0613, n = 8 per group). l, Activation of NTLS→AHN with ChR2 blocked social reward in RES males (EYFP, F (1, 12) = 6.329, P = 0.0271, n = 7, ChR2, F (1, 14) = 0.1567, P = 0.6981, n = 8). m, Inhibition of NTLS→AHN with NpHR rescued social reward deficits in SUS males (EYFP, F (1, 12) = 0.6881, P = 0.4230, NpHR, F (1, 14) = 10.02, P = 0.0069, n = 8 per group). Two-way repeated measures ANOVA were performed for any comparison in this figure: ns, not significant. * P < 0.05, ** P < 0.01. All data are expressed as mean ± s.e.m. BioRender was used to generate schematic figures in a.
