Extended Data Fig. 10: Optogenetic validation of NTLS monosynaptic projections and effects of optogenetic stimulation of non-NTLS neurons on social behaviour.
From: Social trauma engages lateral septum circuitry to occlude social reward
a,b, ChR2-assisted circuit mapping of NTLS→NAc and NTLS→AHN pathways showing monosynaptic (with TTX), inhibitory (Cs-based internal, clamped at 0 mV) and GABAa-dependent (SR-95531, Gabazine) connections in 5/8 NAc/NDB neurons and 4/10 AHN neurons. c, Light-evoked inward current in a ChR2-EYFP+ Cre+ neuron in voltage-clamp mode (clamped at −70 mV) upon 1 s illumination with 470 nm blue light (left) and 15 Hz pulse-induced spikes (right). d, Schematic diagram of non-NTLS neuron infection and manipulation during social behaviour tests. e,f, Multiplex ISH for Nt and CreOff-ChR2-EYFP. c, Overlap of Nt+ EYFP+ neurons among EYFP+ neurons (2-3 slices per mouse, n = 5 mice). g, Light-evoked inward current in ChR2-EYFP+ non-Cre neurons in voltage-clamp mode (clamped at −70 mV) upon 1 s illumination with blue light (470 nm). h, Effect of ChR2 stimulation of non-Nt neurons on social investigation and social avoidance during RI test (Mixed-effects analysis two-way ANOVA, left, F (1, 13) = 0.2137, P = 0.6516, right, F (1, 13) = 0.5672, P = 0.4648, n = ChR2 (10), EYFP (5)). ns, not significant. All data are expressed as mean ± s.e.m. Scale bar, 50 μm.
