Fig. 2: NTLS neuronal activity is associated with altered social behaviours in susceptible mice.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Timeline of iDISCO+ Fos analysis from the RI test after CSDS. Timed sac, mice were perfused 90 min after RI test. b, Mouse brain before and after iDISCO+ clearing. c, Autofluorescence and Fos signal from lightsheet imaging. d, ClearMap analysis showing differentially activated brain regions from RES versus SUS mice. Arrowheads indicate LS. e, Neurotensin expression from Allen Brain Atlas ISH data. f, Timeline of ISH. g,h, Multiplex ISH (g) showing Fos expression (h) in NT neurons in females (one-way ANOVA, F (2, 6) = 7.887, P = 0.0209, n = 3 mice per group, three slices per mouse) and males (F (2, 10) = 13.13, P = 0.0016, n = 3 (CTRL), 4 (RES), 6 (SUS), three slices per mouse); scale bars, 50 μm. LV, lateral ventricle. i, Timeline of slice electrophysiology (ePhys) following CSDS. j, eYFP+ NTLS neurons patched in whole-cell configuration. k, Current–frequency curve showing counts of action potentials evoked by incremental steps of injected current. NTLS neurons from SUS mice (n = 55 neurons) compared with RES mice (two-way ANOVA, P < 0.0001, n = 19). l, Resting membrane potential (RMP) for SUS and RES mice (two-tailed Mann–Whitney test, P = 0.0336, n = 4 (RES), 9 (SUS). m, Correlation between SI ratio and firing rate evoked by a 100 pA step current (Pearson’s correlation, R2 = 0.34, P = 0.0351). Each dot represents the mean value per mouse for RES (red, n = 4) and SUS (black, n = 9) mice. n, Sample traces of excitability for RES (red) and SUS (black) mice following 100 pA current injection. *P < 0.05, **P < 0.01. All data expressed as mean ± s.e.m.
