Fig. 5: Optogenetic manipulation of NTLS downstream regions alters social behaviour in CSDS mice.
From: Social trauma engages lateral septum circuitry to occlude social reward
a, Anterograde AAV-DIO-eYFP tracing from NTLS neurons. b, Anterograde HSV-1 (H129ΔTK-TT) trans-synaptic tracing (70 h post injection) verifies monosynaptic connections between NTLS neurons and regions shown in a. c, AAV-DIO-ChR2 injection and timeline for resident intruder optogenetics experiments. d–l, ChR2 axon terminal activation in NAc (d), AHN (g) and PAG (j) during RI test in females (NAc (e), social investigation, F (1, 12) = 4.836, P = 0.0482; social avoidance, F (1, 12) = 2.935, P = 0.1123, n = 8 (ChR2), 6 (eYFP); AHN (h), social investigation, F (1, 12) = 4.947, P = 0.0461, social avoidance, F (1, 12) = 0.8571, P = 0.3728, n = 8 (ChR2), 7 (eYFP)); PAG (k), social investigation, F (1, 13) = 0.6986, P = 0.4183; social avoidance, F (1, 13) = 0.07324, P = 0.7909, n = 8 (ChR2), 6 (eYFP); and in males (social investigation, NAc (f), F (1, 13) = 4.540, P = 0.0528; social avoidance, F (1, 13) = 0.2848, P = 0.6026, n = 9 (ChR2), 5 (eYFP); AHN (i), social investigation, F (1, 13) = 28.94, P = 0.0001, social avoidance, F (1, 13) = 0.06521, P = 0.8024, n = 8 (ChR2), 7 (eYFP); PAG (l), social investigation, F (1, 14) = 0.002038, P = 0.9646; social avoidance, F (1, 14) = 1.750, P = 0.2071, n = 9 (ChR2), 6 (eYFP) (f)). Two-way repeated-measures ANOVA was performed for all comparisons. *P < 0.05, **P < 0.01, ****P < 0.0001. All data expressed as mean ± s.e.m. Scale bars, 200 μm. The illustrations in c were created with BioRender (https://biorender.com).
