Extended Data Fig. 5: Distinct tumor cell populations in primary and metastatic medulloblastomas.
From: RETRACTED ARTICLE: Human fetal cerebellar cell atlas informs medulloblastoma origin and oncogenesis
a, Scatter plots of mixed G3 and G4 MB tumor cells based on Group 3/4-B/C metaprogram expression scores. b, Heatmap of CNV signals normalized against the “Microglia” cluster shows CNV changes by chromosome (columns) for individual cells (rows) based on scRNA-seq data. c-d, UMAP of merged cells (c), split plot of cell populations (d), cell compositions (e) from the BT-325 primary and metastatic tumours. f, Two-dimensional cell-state plots and proportions of tumor subpopulations in BT-325 primary and metastatic MB. Each vertex corresponds to one key cellular state along the transformation. The colors represent the count. g, Dot plot displaying the expression of selected marker genes in the primary and metastatic tumors. The dot size reflects the percentage of the cells that express the gene. Average expression levels are color coded. h, CNV analysis of chromosome 8 based on DNA methylation showing the MYC gene amplification in the metastatic tumor of BT-325. i, Dot plot showing the expression levels of G3 and G4 marker genes in the BT-325 primary (x-axis) and metastatic (y-axis) tumors. Red and green indicate higher and lower expression, respectively, in the metastatic tumor compared to the primary tumor. j, UMAP of merged cells from the G3 MB (#2) primary and metastatic tumors (left). Split plot (middle and right) of cell populations from primary and metastatic samples. The cells are colored by cell type. k, Expression of indicated TCP markers and MYC in the cells from the G3 MB (#2) primary and metastatic tumors. l, m, Representative images (l) and the quantification (m) of four paired primary and metastatic human G3 MB tissues stained for HNRNPH1 and SOX11 (n = 4 individual patients). Scale bars: 50 μm. Unpaired two tailed t-test.
