Extended Data Fig. 6: Reference mapping of single-nuclei chromatin accessibility from G3 and G4 MBs.
From: RETRACTED ARTICLE: Human fetal cerebellar cell atlas informs medulloblastoma origin and oncogenesis
a, Workflow for integrating scRNA-seq and snATAC-seq data analysis using ArchR. b,c, Comparation of the cell types in the b) G3 and c) G4 MB tumor tissues detected by scRNA-seq (upper) and snATAC-seq (lower) using Seurat and Signac analysis. d,e, The gene integration matrix from scRNA-seq and snATAC-seq (left panel) and alignment for key TCP- and MB-related marker genes (right panels) in d) G3 MB samples (n = 2 patients), and e) G4 MB samples (n = 3 patients). The individual cell types are indicated by colors. The integrated gene activity of G3 or G4 signature genes is shown in the right panels. f, Heatmap showing correlation of accessibility of promoter regions with target gene expression for tumor cell types. g, Pseudo-bulk ATAC-seq tracks at the indicated gene loci in G3 and G4 MB cells.
