Extended Data Fig. 1: Schematic diagrams of the constructs used in this study.
From: Molecular basis for selective activation of DREADD-based chemogenetics

a-b, Modified snake diagrams of hM3Dq and hM4Di from the GPCRdb including the N-terminal and ICL3 truncation sites. The LgBit was fused at the C terminus of the hM3Dq receptor. DREADD mutation sites are indicated by red circles. Residues in yellow circles were removed from the expression constructs. c, Schematic diagram of the 3-in-1 vector (pFastDual plasmid-based) for the expression of miniGq or miniGo. The HiBit was fused at the C-terminus of the Gβ subunit. d-e, The activities of intact and ICL3 deletions of hM3Dq (d) or hM4Di (e) under the stimulation by DCZ, respectively. See Supplementary Table 4 for fitted parameter values that represent the mean ± SEM of n = 4 biologically independent experiments. f-g, Surface expression levels of intact and ICL3 deletions of hM3Dq and hM4Di. The WT hM3R and hM4R are also included as controls. Data represent the mean ± SEM of n = 3 biologically independent experiments. See Supplementary Table 11 for the details.