Extended Data Fig. 2: CryoEM analysis for the complexes of DCZ-bound hM3Dq-miniGq, DCZ-bound hM4Di-miniGo, CNO-bound hM3Dq-miniGq, and iperoxo-bound hM3R-miniGq.
From: Molecular basis for selective activation of DREADD-based chemogenetics
For each of the respective complexes are shown: a, Selected frame aligned micrograph. The experiment was repeated three times with similar results. b, Histograms of defocus values for micrographs used in the single-particle analysis (see Extended Data Table 1 for more details). c, Orientational distribution heat map. d, 2D plots of the gold-standard Fourier shell correlation (GSFSC) between half maps (black) and FSC between model and the b-factor sharpened map for respective refined model (red) as calculated by phenix.mtirage. Local refinement GSFSC is shown in gray and the locally refined map to the model is shown in plumb. e, Local resolution heat-map calculated using the local windowed FSC method.
