Extended Data Fig. 3: Overall structure and CHS binding site of the iperoxo bound hM3R-miniGq complex.
From: Molecular basis for selective activation of DREADD-based chemogenetics
a, Superposition of the overall structures of the iperoxo-bound hM3R-miniGq and DCZ-bound hM3Dq-miniGq. The density map of the iperoxo is shown as gray meshes. b-c, The extracellular (b) and intracellular (c) views of structural comparison of the receptors between hM1R-G11 (PDB: 6OIJ) and hM3Dq-miniGq complexes, respectively. d-e, The extracellular (d) and intracellular (e) views of structural comparison of the receptors between hM3Dq-miniGq and hM4Di-miniGo complexes, respectively. f, Comparison of the interfaces between TM5 of hM1R or hM3Dq and α5 helix of G11 or miniGq subunit in the structures of hM1R-G11 or hM3Dq-miniGq complex. g, Three views of the superposition of hM1R (6OIJ) and hM3R structures. The CHS molecules in the hM1R or hM3R structure are shown in gray or yellow spheres, respectively.
