Extended Data Fig. 1: Cell type specific ribosome: Ribosomal proteome profiles and RP identification from 80S monosomes across mouse tissues and different stages of postnatal development testis.
From: A male germ-cell-specific ribosome controls male fertility
a, Separation of cytoplasmic ribosomes through sucrose gradient fractionation using different adult mouse tissues. b, Venn diagram of the identified RPs in a comparative analysis of this and earlier studies. c, Distribution of RP paralogues in different mouse tissues identified in this study. d, Correlation matrix of cytoplasmic ribosomes from different mouse tissues. e, Density plot of maximal SPM of RPs of nine mouse tissues. f, The relationship between retrogene Rpl39l and its X-linked parental gene Rpl39. g, mRNA levels of Rpl39 and Rpl39l in 15 mouse tissues. n = 3. h, Relative quantification of RPL3L in nine mouse tissues using a PRM method. n = 3. i, Separation of cytoplasmic ribosomes through sucrose gradient fractionation using mouse testicular tissue at different postnatal developmental stages. j, Correlation matrix for cytoplasmic ribosomes from mouse testicular tissue at different developmental stages. k, Relative protein quantification of RPL4 and RPL39L in non-ribosome, 40S, 60S and ribosome fractions by PRM. n = 3. l, Morphology and specific staining of mouse testicular cells: testicular interstitial cells (IC), Sertoli cells (SC), spermatogonia (SG), pachytene spermatocytes (PS), round spermatids (RS), and elongated spermatids (ES). Scale bar, 20 μm. m, Purities of SG (82.60 %), PS (82.69 %), RS (87.76 %), ES (81.9 %), IC (92.63 %) and SC (96.35 %) cells. n = 3. n, Quantification of Rpl39 and Rpl39l mRNA levels in testicular cells by qPCR. n = 3. o, Absolute protein quantification of RPL10, RPL10L, and RPL22, RPL22L1 in testicular cells by PRM. n = 3. For g, h, k, m, n and o, data are mean ± s.e.m. n values represent the number of samples.
