Extended Data Fig. 5: ACE2 and Domain B dependent entry of NeoCoV, PDF-2180 S pseudotyped viruses in different cell types.
From: Close relatives of MERS-CoV in bats use ACE2 as their functional receptors
a-c, BHK-21, HEK293T, Vero E6, A549, Huh-7, and Tb 1 Lu cells were transfected with either Bat40ACE2 or Bat40DPP4. Expression, pseudotyped virus entry (GFP) (a), and luciferase intensity (b-c) were detected at 16 h post-infection. Data are presented as mean ± SD (biological triplicates). Data representative of two independent transfection and infection assays. d, Expression levels of the indicated chimeric viral S glycoproteins (anti-HA) in HEK293T whole cell lysates. GAPDH was used as a loading control. e, Chimeric viral S glycoproteins (anti-HA) and VSV-M level in pseudotyped viruses precipitated from virus-containing supernatant. Blots representative of two independent transfection assays for pseudovirus production.
