Extended Data Fig. 2: Bat ACE2 orthologs tested in this study and their ability to support the entry of NeoCoV and PDF-2180.
From: Close relatives of MERS-CoV in bats use ACE2 as their functional receptors
a, Phylogenetic tree and assessment of the ability of a panel of ACE2 orthologs from 46 bat species to support NeoCoV and PDF-2180 S-mediated entry (relative to Bat40 ACE2). ACE2 orthologs deficient in supporting the entry of both viruses are highlighted in red. The GenBank accession numbers and protein sequences were summarized in Supplementary Table 4. b, Immunofluorescence assay detecting the 46 bat ACE2 orthologs C-terminal 3×Flag tag in the HEK293T stable cells. Mock indicates cells were transduced with lentiviruses produced by empty vector only. Data representative of three independent immunostaining assays with similar results.
