Extended Data Fig. 7: Representative cryo-EM densities.
From: RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2
a, target RNA and crRNA from ternary complex map, showing 5′ crRNA handle and 3′ target RNA PFS. b, 5′ handle and 7-nt pre-ordered seed from Cas12a2 binary complex. c, Cleaved strand pair of aromatic clamps. d, non-cleaved strand (NCS) aromatic clamps. Density for the unwound bases of the NCS is diffuse due to flexibility, but the nucleobase held within the aromatic clamp is well-resolved. e, RuvC active site, including density for scissile phosphate, two Mg2+ ions and a putative activating water.
