Fig. 3: LC4 synaptic number gradients onto DNp02 and DNp11 are functional.
From: Synaptic gradients transform object location to action
a, Whole-cell electrophysiological recordings of DNp02 (red) and DNp11 (blue) to looming stimuli at 32.5° (for DNp02) and 70° (for DNp11) in azimuth. Looming stimulus is an array of three discs expanding 0° to 30° diameter at 500° s−1. Shown are representative traces from a single fly and stimulus. b, Representative responses from a single fly for 32.5° (top) and 70° (bottom) azimuth looming stimuli. c, Spike raster plots of DN responses during the 150 ms after looming onset. Coloured trials show the traces in b. d, Averaged response of the traces in b shows subthreshold depolarizing responses to looming stimuli. Shaded area, estimated depolarization from the baseline. e, Mean per-trial spike count across individual flies (from c). n, individual trials; **P < 0.01. f, Pooled mean of integrated potentials across individual flies. n, individual trials. Repeated-measures one-way ANOVA, Dunnett’s test. Error bars, s.e.m.; **P < 0.01, ***P < 0.001, see Supplementary Table 1 for exact P values. g, Mollweide projection of estimated dendritic receptive fields for all 55 LC4 neurons in the FAFB EM dataset. Polygons are estimated visual fields of individual LC4 neurons (example individual fields in red and blue). h, DNp02 and DNp11 LC4-receptive fields estimated on the basis of summed input from individual LC4 fields in g. i, In vivo whole-cell (dashed) and model-estimated (solid) DN responses to three-loom-array stimuli (solid). j, Estimated DNp02 and DNp11 responses to modelled three-loom-array stimuli across the whole visual hemifield, based on receptive fields in h.
