Extended Data Fig. 8: Supplemental data for infant rabbit infections.
From: Undecaprenyl phosphate translocases confer conditional microbial fitness
(a): Total CFU in mixed infections with differing inoculum pH (n = 6 each for pH 7 and 9). (b): Fluid accumulation ratio (FAR) in mixed infections with differing inoculum pH. (c): Representative small intestinal CFU plates from single infections showing transmission of WT V. cholerae (greyish translucent colonies) from WT-infected rabbits to Δvca0040-infected rabbits in the same litter. Note that Δvca0040 colonies (white arrows) are lighter and smaller than WT colonies, making them visually distinguishable and countable. (d): Direct imaging of GFP+ V. cholerae in single infection CF samples. Large GFP-bright spheres are likely Δvca0040 cells in the presence of transmitted, rod-shaped WT cells. Scale bars, 3 μm. a: geometric means analyzed with two-tailed Mann-Whitney U tests. b: mean ± SD analyzed with an unpaired student’s two-tailed t-test. c, d: representative images from n = 3 WT and n = 7 Δvca0040 singly-infected rabbits.
