Fig. 2: DUF368 function is conserved and necessary for peptidoglycan maintenance.
From: Undecaprenyl phosphate translocases confer conditional microbial fitness
a,b, Total peptidoglycan (a) and intracellular UDP-M5 (b) in V. cholerae grown in M63 minimal medium at the indicated pH. Suppressor, Δvca0040/ΔsecDF1. c, Alkaline growth of ΔSAOUHSC_00846 (Δ0846) S. aureus in the indicated conditions and with the indicated expression vectors. Representative results from n = 3 independent experiments per condition. d,e, Total peptidoglycan (d) and intracellular UDP-M5 (e) in S. aureus grown in tryptic soy broth (TSB) with 100 mM bicine at the indicated pH. a,b,d,e, n = 3 cultures per strain or condition; data are mean ± s.d. a,b, One-way ANOVA with Tukey’s multiple comparison test. d,e, Unpaired Student’s two-tailed t-test.
