Fig. 3: C55-P recycling is impaired in bacteria lacking DUF368-containing proteins.
From: Undecaprenyl phosphate translocases confer conditional microbial fitness
a, Fold change (FC) in MIC for ΔSAOUHSC_00846 S. aureus in the indicated conditions. Amphomycin*, amphomycin with 1 mM CaCl2. b, Ultra high performance liquid chromatography (UPLC) traces of purified C55-OH (a) and C55-P (b) standards (top), wild-type S. aureus lipid extracts spiked with each standard (middle) or wild-type S. aureus treated with 50 μg ml−1 amphomycin (bottom). Rt, retention time. c, UPLC traces of lipid extracts of wild-type or mutant S. aureus grown in TSB pH 7 (top) or pH 8.5 (bottom). d,e, Relative abundance of C55-OH (d) and C55-P (e) under same conditions as in c. f, UPLC trace of lipid extracts of complemented ΔSAOUHSC_00846 S. aureus grown in TSB pH 8.5. g,h, Raw peak proportions of C55-OH (g) and C55-P (h) in each strain grown at pH 8.5. i, S. aureus stained with ampho-FL at pH 8.5. Scale bars, 5 μm. DIC, differential interference contrast image. j, Quantification of ampho-FL signal. Symbols represent the mean ampho-FL intensity of 1 to 8 individual bacterial clusters in independent cultures. k, Proposed model of disrupted C55-P recycling and the associated consequences in bacteria lacking C55-P translocase activity. b,c,f,i, Representative data from n = 3 cultures per strain or condition. d,e,g,h,j, Data are mean ± s.d. from n = 3–5 cultures per strain or condition. Each point is an independent culture. d,e, Unpaired student’s two-tailed t-test. g,h,j, One-way ANOVA with Tukey’s multiple comparison test.
