Fig. 5: DUF368 is required for V. cholerae pathogenesis.
From: Undecaprenyl phosphate translocases confer conditional microbial fitness
a,b, Schematic (a) and intestinal competitive indices (b) in mixed-infection models (n = 6 rabbits in each group). SI, small intestine. c–e, Schematic (c), number of intestinal colony-forming units (CFU) (d) and fluid accumulation ratio (FAR) (e) in single infections (n = 3 rabbits (wild type) and n = 8 rabbits (Δvca0040)). CF, caecal fluid; pSI, proximal small intestine; mSI, medial small intestine; dSI, distal small intestine. Open circles indicate rabbits with limit of detection (LOD) measurements where the true CFU burden is at least (for upper LOD circles) or at most (for lower LOD circles) the plotted value. Note two Δvca0040 animals had insufficient caecal fluid accumulation for FAR calculation and were assigned LOD values (arbitrary 25 μl volume). b,d, Data are geometric mean. d, Two-tailed Mann–Whitney U tests. e, Data are mean ± s.d. analysed with an unpaired Student’s two-tailed t-test. f, pH of caecal fluid from infected animals. Data are mean ± s.d. g, Incubation of laboratory-grown V. cholerae with filtered caecal fluid samples from three different rabbits. Representative images from n = 3 ex vivo incubations with independent caecal fluid samples. Scale bars, 3 μm. h, Proposed model for microbial C55-P translocation and its integrated control by environmental inputs, the presence of other translocases, and non-translocase-related environmental adaptation mechanisms.
