Extended Data Fig. 1: Preparation and characterization of the NLRP3-NEK7 complex.
From: Cryo-EM structures of the active NLRP3 inflammasome disc
a, Gel filtration profile of the NLRP3-NEK7 complex showing the two peaks. b, SDS-polyacrylamide gel electrophoresis (PAGE) of gel filtration fractions for the NLRP3-NEK7 complex. Locations of the two component proteins are labeled. c, Representative negative-staining images from the two NLRP3-NEK7 complex peaks. d, Gel filtration profile of the NLRP3-NEK7 complex with ATPγS showing one major peak. e, SDS-PAGE of the gel filtration fractions for the NLRP3-NEK7 complex with ATPγS. Locations of the two component proteins are labeled. f, A representative negative-staining image from the peak containing NLRP3, NEK7 and ATPγS. g, A representative cryo-EM image from the peak containing NLRP3, NEK7 and ATPγS. Experiments in (a-g) were repeated at least three times. h, 2D classes from cryo-EM images of the NLRP3-NEK7 complex with ATPγS. No full disk was observed.
