Extended Data Fig. 2: Preparation and characterization of NLRP3-NEK7-ASC inflammasome complexes.
From: Cryo-EM structures of the active NLRP3 inflammasome disc
a, Gel filtration profile of the NLRP3-NEK7-ASC complex. b, SDS- PAGE of the near void peak from the gel filtration chromatography. Locations of the three component proteins are labelled. c, Mass spectrometry that detected ASC PYD in the band in (b). d, A representative cryo-EM image from a sample containing NLRP3, NEK7, ATPγS, and 1:1 ASC PYD. Experiments in (a-b) and (d) were repeated at least three times.
