Extended Data Fig. 6: Single-cell analysis by FIND-seq.
From: Identification of astrocyte regulators by nucleic acid cytometry
(a) Visualization of a single droplet in flat-bottom microwell plates. Refraction of light at well edges obscures imaging. This is solved by sorting directly into hexadecane. HFE oil sinks, forming a convex shape that forces droplets to the center so that they can be imaged. (b) The percentage of reads mapping to mouse or human cells after single-cell sorting cell mixtures. Mouse (3T3) and human (JLat) cells were mixed 1:100, sorted based on a TaqMan PCR targeting JLat cells, and the transcriptome was sequenced. (c) Quality control analyses for scFIND-seq. (d) Marker genes of Aqp4+Edem1+/– cells from naïve or EAE mice analyzed by scFIND-seq. (e) Elbow plot of principal components detected by scFIND-seq.
