Extended Data Fig. 8: Control analyses of Nr3c2 and Ncor2 knockdown.
From: Identification of astrocyte regulators by nucleic acid cytometry
(a) Left: Upstream regulator analysis of RNA-seq data by Qiagen IPA from sgNr3c2-targeted versus sgScrmble-targeted mice shows NR3C2 downregulation. Fisher’s exact test. Right: Validation of NR3C2 knockdown by immunostaining quantification. n = 6 images per group from n = 3 mice. Unpaired two-sided t-test. (b–c) FACS analysis of total CD4+ cells or FoxP3+, IFN𝛾+, IL17+, and IL10+ CD4 cell subsets in the (b) the spleen and (c) CNS. (d) Validation of NCOR2 knockdown by immunostaining. n = 6 images per group from n = 3 mice. Unpaired two-sided t-test. (e–f) FACS analysis of total CD4+ cells or FoxP3+, IFN𝛾 +, IL17+, and GM-CSF+ CD4 cell subsets in the (e) the spleen and (f) CNS.
