Extended Data Fig. 2: Droplet formation and capture of mRNA by FIND-seq.
From: Identification of astrocyte regulators by nucleic acid cytometry
(a) Schematic of bubble trigger device that drives air-triggered droplet formation during cell encapsulation. (b) Air-triggered droplet formation enables kHz generation of beads from viscous molten agarose. Images from time-lapse videos of agarose jet breakup. (c) Functionalization of agarose with allyl groups is used to directly link acrydited primers. (d) Microscope images demonstrating successful conjugation of polyT primers to agarose with polyA-FAM probes. (e) Quantification of agarose-bound polyT.
