Extended Data Fig. 10: Processing pipeline of the rabbit ribosome with recombinant zebrafish Dap1b.
From: A molecular network of conserved factors keeps ribosomes dormant in the egg
a, Processing pipeline for obtaining an 80S density map of the rabbit ribosome with zebrafish Dap1b. b, Orientation distribution plot (top) and Gold-Standard Fourier Shell Correlation (GSFSC; bottom) of Map1. c, Local resolution maps calculated for Map2 and Map3. Densities and local resolutions of eIF5A, Dap1b, eEF2 and SERBP1 are shown on the bottom. Note that the resolution of the small subunit in Map2 is 0 (shown in blue) since this region was outside the mask used for generating this map. d, Latent space representation of particles from rabbit ribosomes with zebrafish Dap1b as a UMAP embedding after training a cryoDRGN latent variable model. Classes are depicted in Roman numbers, map volumes are indicated with Arabic numbers. Total particle number is shown on the top left of the graph.
