Extended Data Fig. 1: Mass-spectrometry and cryo-EM analysis of Xenopus egg ribosomes.
From: A molecular network of conserved factors keeps ribosomes dormant in the egg
a, Fold change of ribosome-associated factors and core ribosomal proteins after comparing mass spectrometry data of purified ribosomes from unfertilized Xenopus eggs and 24 hpf larvae (stage 14) (n = 1 experiment). b, Processing pipeline of the Xenopus egg ribosome. All steps were done in Cryosparc v3.2.0. Maps are shown in grey, masks in blue. c-d, Maps showing the local resolution of Map1 and Map2 (c), and of the four factors associated with Xenopus egg ribosomes (d). Note that the resolution of the large subunit in Map2 (in c) is 0 (shown in blue) since this region was outside the mask used for obtaining this map (see b). e, Orientation distribution plot for all particles contributing to Map1. f, Gold-Standard Fourier Shell Correlation (GSFSC) of Map1.
