Extended Data Fig. 2: Processing pipeline of the 1 hpf zebrafish ribosome.
From: A molecular network of conserved factors keeps ribosomes dormant in the egg
All steps were done in Cryosparc v3.2.0. Maps are shown in grey, masks in blue. The orientation distribution plot for all particles contributing to Map1 and the Gold-Standard Fourier Shell Correlation (GSFSC) of the respective map is shown on the bottom. Local resolution maps were calculated for Map1, Map2, Map3, and for the four ribosome-associated factors. Note that in the box with local resolution maps, the resolution of the large subunit in Map2 and Map3, and of the small subunit’s head in Map3 is 0 (shown in blue) since these regions were outside the masks used for generating these maps.
