Extended Data Fig. 3: Characterization of the dormant ribosome state in zebrafish 1 hpf and Xenopus egg ribosomes.
From: A molecular network of conserved factors keeps ribosomes dormant in the egg
a-d, Densities of the two modules, namely Habp4-eEF2b/eEF2 (a-b), and Dap1b/Dapl1-eIF5a (c-d), that are characteristic for dormant ribosomes in zebrafish 1 hpf embryos and Xenopus eggs. e, Overview of the ribosome structure isolated from 6 hpf zebrafish embryos lacking the specific egg ribosome-associated factors. f, Latent space representations of ribosomal particles from Xenopus eggs (left), 1 hpf zebrafish embryos (middle) and 6 hpf zebrafish embryos (right) as UMAP embeddings after training a cryoDRGN latent variable model. Classes are depicted in Roman numbers, map volumes are indicated with Arabic numbers. Total particle numbers are shown on the top left of each graph. g, Densities of ribosome-associated factors from Xenopus eggs. An overview of the map is shown on the top left. h, Densities of ribosome-associated factors in a map reconstructed from class I particles from Xenopus eggs obtained with cryoDRGN. An overview of the map is shown on the top-left.
