Extended Data Fig. 7: Myriocin improves innervation in the context of serine deficiency and alters the hepatic lipidome.
From: Insulin-regulated serine and lipid metabolism drive peripheral neuropathy
(a) Representative images of intraepidermal nerve fiber (IENF) density in mice fed a with low fat diet + vehicle (LFD+Veh, n = 10), high fat diet + vehicle (HFD+Veh, n = 7), serine/glycine free HFD + vehicle (-SG HFD+Veh, n = 12), and -SG HFD + myriocin (-SG HFD+Myr, n = 8). (b) Representative images and quantification of corneal nerves in mice fed a with low fat diet + vehicle (LFD+Veh, n = 12), high fat diet + vehicle (HFD+Veh, n = 12), serine/glycine-free HFD + vehicle (-SG HFD+Veh, n = 12), and -SG HFD + myriocin (-SG HFD+Myr, n = 11). (c) Tactile sensing in mice fed with a low fat diet + vehicle (LFD+Veh, n = 12), high fat diet + vehicle (HFD+Veh, n = 12), serine/glycine-free HFD + vehicle (-SG HFD+Veh, n = 12), and -SG HFD + myriocin (-SG HFD+Myr, n = 11). (d) Motor nerve conduction velocity in mice fed with a low fat diet + vehicle (LFD+Veh, n = 12), high fat diet + vehicle (HFD+Veh, n = 12), serine/glycine-free HFD + vehicle (-SG HFD+Veh, n = 12), and -SG HFD + myriocin (-SG HFD+Myr, n = 11). (e) Liver high-resolution lipidomics analysis in the fed state in mice fed with a low fat diet + vehicle (LFD+Veh, n = 12), high fat diet + vehicle (HFD+Veh, n = 12), serine/glycine-free HFD + vehicle (-SG HFD+Veh, n = 12), and -SG HFD + myriocin (-SG HFD+Myr, n = 11). Data are mean ± standard error of mean (SEM) and were analyzed using a one-way ANOVA with Fisher’s LSD post hoc test (b–e).
