Extended Data Fig. 8: Myriocin slows the progression of peripheral neuropathy in BKS-db/db mice.
From: Insulin-regulated serine and lipid metabolism drive peripheral neuropathy
a) Hydrolyzed plasma deoxysphinganine (deoxySA) concentration in 16-week-old WT and BKS-db/db mice (n = 6 per group). b) Hepatic targeted sphingolipid determination 16-week-old WT and BKS-db/db mice (n = 6 per group). c) Thermal latency time-course in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (0.3 mg/kg every other day, n = 9) for 8 weeks. d) Tactile sensing in BKS-db/db mice treated with either vehicle (n=10) or myriocin (n = 9) for 8 weeks. e) Intraepidermal nerve fiber density in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. f) Body weight time-course in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. g) Fasting (6-h) blood glucose time-course in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. h) Fasting (6-h) plasma serine time-course in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 8) for 8 weeks. i) Summed hepatic sphingolipids in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. j) Summed paw skin sphingolipids in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. k) Stack plot of liver deoxydihydroceramide (deoxyDHCer) species in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. l) Stack plot of paw skin deoxydihydroceramide (deoxyDHCer) species in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. m) Stack plot of liver sphingomyelin (SM) species in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. n) Stack plot of paw skin sphingomyelin (SM) species in BKS-db/db mice treated with either vehicle (n = 10) or myriocin (n = 9) for 8 weeks. Data are mean ± standard error of mean (SEM) and were analyzed using a two-sided independent t-test (a–b,d–e, i–n) and a two-way ANOVA with Fisher’s LSD post hoc test (c,f–h). Statistical analyses in k-n were performed using summed sphingolipid abundances.
