Fig. 2: Dietary serine restriction suppresses fatty acid synthesis and mitigates adiposity.

a, Plasma serine levels in fed and overnight-fasted mice fed with LFD, −SG LFD, HFD or −SG HFD for 18 weeks (n = 10 per group). b, Plasma glycine levels in mice fed with LFD, −SG LFD, HFD or −SG HFD for 18 weeks (n = 10 per group). c, Body weight of mice fed with LFD, −SG LFD, HFD, or −SG HFD (n = 13 per group). d, Body composition in mice 18 weeks after feeding with LFD (n = 10), −SG LFD (n = 12), HFD (n = 13) or −SG HFD (n = 13). e, Glucose tolerance test (GTT) AUC 18 weeks after feeding with LFD, −SG LFD, HFD or −SG HFD (n = 13 per group). f, Insulin tolerance test (ITT) AUC 18 weeks after feeding with LFD, −SG LFD, HFD or −SG HFD (n = 15 per group). g, Phylogenetic alpha diversity in mice fed with LFD, −SG LFD, HFD or −SG HFD (n = 10 per group). h, Log fraction of species from the fatty acid synthesis pathway in mice fed with LFD (n = 10), −SG LFD (n = 10), HFD (n = 9) or −SG HFD (n = 9) for 18 weeks. i, Hepatic de novo palmitate synthesis in mice fed with LFD (n = 3), −SG LFD (n = 4), HFD (n = 5) or −SG HFD (n = 5) for 18 weeks. j, Thermal sensing in mice fed with LFD (n = 15), −SG LFD (n = 15), HFD (n = 14) or −SG HFD (n = 15 per group). Data are mean ± s.e.m. and minimum and maximum (g,h), and were analysed using two-way ANOVA with Fisher’s least significant difference post hoc test (a–j).